Notch3 Knockout cell line (B16-F10)

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location： Home > Products > KO Cell Lines > Notch3 Knockout cell line (B16-F10)

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Notch3 Knockout cell line (B16-F10) Order

Notch3 Knockout cell line (B16-F10)
Catalog#
YKO-M218
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Notch3 Knockout cell line (B16-F10)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Notch3 Knockout cell line (B16-F10)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Gene Name
Notch3
Gene ID
18131
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 4 (based on transcript Notch3-201)
Validation: PCR+Sanger Sequencing
Notch3 Knockout cell line (B16-F10) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:B16-F10		 STR Info (Cell bank)
Cell Line:B16-F10
Allele1Allele2Allele3Allele1Allele2Allele3
4-2 237.03
【20.3】		241.13
【21.3】		20.321.3
5-5 343.51
【16】		359.57
【20】		16.020.0
6-4 299.35
【18】		303.47
【19】		18.019.0
6-7 345.99
【15】		15.0
9-2 232.84
【18】		236.94
【19】		18.019.0
12-1 229.14
【17】		233.26
【18】		17.018.0
15-3 200.43
【22.3】		204.54
【23.3】		22.323.3
18-3 147.05
【15】		151.1
【16】		15.016.0
X-1 411.87
【28】		28.0
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Notch3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the B16-F10 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Notch3 gene knockout Notch3 Knockout cell line (B16-F10) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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